Journal: Beilstein Journal of Organic Chemistry
Article Title: Activity assays of NnlA homologs suggest the natural product N -nitroglycine is degraded by diverse bacteria
doi: 10.3762/bjoc.20.75
Figure Lengend Snippet: Molecular mass determination of purified NnlA homologs by A) SDS-PAGE or B) analytical size exclusion chromatography. Homolog labeled in figure. Mobile phase and sample buffer is 100 mM tricine 100 mM NaCl buffer at pH 7.5. Dashed grey lines represent elution volumes of molecular mass standards. Theoretical molecular weights are as follows Vs NnlA: 21,869 Da; Mr NnlA: 20638 Da; Pd NnlA: 18,473 Da; Ms NnlA : 18,239 Da; and Ps NnlA: 19,042 Da.
Article Snippet: Protein size exclusion chromatography standards (BioRad) were used to determine molecular masses.
Techniques: Purification, SDS Page, Size-exclusion Chromatography, Labeling